Capillary Tube Assay for Staphylococcal Enterotoxins A, B, and C
نویسندگان
چکیده
منابع مشابه
Thermal inactivation of staphylococcal enterotoxins B and C.
Thermal inactivation profiles of staphylococcal enterotoxins B (SEB) and C (SEC) at 80, 100, and 121 C showed that SEC is more resistant than SEB to heat. After 24 h of incubation at 25 C, some reactivation (recovery of serological reactivity) occurred in toxins that had been inactivated by heat. If the toxin was stirred during heating, reactivation did not occur. An examination of the reactiva...
متن کاملComparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D.
We compared four versions of the enzyme-linked immunosorbent assay for their suitability for detecting staphylococcal enterotoxins. The sandwich with labeled antibody proved to be the best. We used it with a sorbent consisting of antibody-coated polystyrene spheres reacted with 20 ml of food extract. The sensitivity of the test was 0.1 ng of enterotoxin per ml, which is far below clinical relev...
متن کاملPolyvalent antiserum agar system for the detection of staphylococcal enterotoxins A, B, C, and E.
A polyvalent antiserum agar system in capillary tubes was developed and evaluated for the detection of enterotoxins A, B, C, and/or E present in culture supernatant fluids.
متن کاملStaphylococcal Enterotoxins
Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...
متن کاملStaphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1971
ISSN: 0003-6919
DOI: 10.1128/aem.21.3.559-561.1971